ICAN Omics Lipidomics

State-of-the-art technologies such as mass spectrometry coupled with gas or liquid chromatography allow us to guarantee quantitative and ultra-sensitive analyzes on hundreds of lipid species in various biological matrices.

Expertise and services

1. Complex profiling by LC-ESI-MS/MS

Glycerophospholipids (238): PC, LPC, PE, LPE, plasmalogen PE, PI, PG, LPG, BMP, PS, PA
Sphingolipids (70): Sphingomyelin (SM), ceramides, dihydroceramide (DHC), acylceramides
Neutral lipids (135): TAG, DAG, MAG, CE, cholesterol

2. Targeted approach for studies of specific metabolic pathways (LC-MS/MS and GC-EI/CI-MS)

Metabolites from the microbiota: stanols (coprostanol, epicoprostanol, ethylcoprostanol), bile acids (primary, secondary, conjugated)
Free fatty acids (FFA) (27 lipids): 15:0, 16:0, 16:1, 17:0, 18:0, 18:1, 18:2, 18:3 (w3 and w6), 20: 0, 20:1, 20:2, 20:3 (w3 and w6), 20:4, 20:5, 22:0, 22:1, 22:4, 22:6, 23:0, 24:0 , 24:1
Cholesterol synthesis pathway: squalene, lanosterol, desmosterol, lathosterol, cholesterol
Intestinal absorption markers: phytosterols (campesterol, β-sitosterol, stigmasterol), cholestanol

Matrices: Biofluids (plasma, serum, lipoproteins (VLDL, LDL HDL)), faeces, tissue (liver, adipose tissue, brain, heart, muscle), others (exosomes, mitochondria, cells, bacteria, etc.)

Equipment on the platform

GC-MS: Trace 1310/ISQ LT (ThermoFisherScientific)
LC-MS/MS: HPLC (Prominence Shimadzu)/ QTrap 4000 (AB Sciex)
Pre-analytical automaton LV200 (Biotage)
Precellys Evolution homogenizer (Ozyme),
Positive pressure manifold (Waters positive pressure manifold – 96)
SPD131DDA speed vacuum concentrator with cryogenic trap (Thermo Fisher Scientific).

Current developments

Sphingosines, sphingosine-1-Phosphate
Oxysterols

Lipidomics Platform Interaction Radiation

Education: reference platform for the OMICS course of the Biomics master’s degree from Université Paris Est Créteil
Local networks: UMS RésOmique SU
National networks: MetaboHub, RFMF, Lipidomystes, INSERM Microbiota Consortium
International networks: ELM, MPF, metabomeeting, european regional metabolomics networks