- Home
- Scientific platforms
- ICAN Omics
- ICAN Omics Lipidomics
ICAN Omics lipidomics

State-of-the-art technologies such as mass spectrometry coupled with gas or liquid chromatography allow us to guarantee quantitative and ultra-sensitive analyzes on hundreds of lipid species in various biological matrices.



Expertise and services
1. Complex profiling by LC-ESI-MS/MS
- Glycerophospholipids (238): PC, LPC, PE, LPE, plasmalogen PE, PI, PG, LPG, BMP, PS, PA
- Sphingolipids (70): Sphingomyelin (SM), ceramides, dihydroceramide (DHC), acylceramides
- Neutral lipids (135): TAG, DAG, MAG, CE, cholesterol
2. Targeted approach for studies of specific metabolic pathways (LC-MS/MS and GC-EI/CI-MS)
- Metabolites from the microbiota: stanols (coprostanol, epicoprostanol, ethylcoprostanol), bile acids (primary, secondary, conjugated)
- Free fatty acids (FFA) (27 lipids): 15:0, 16:0, 16:1, 17:0, 18:0, 18:1, 18:2, 18:3 (w3 and w6), 20: 0, 20:1, 20:2, 20:3 (w3 and w6), 20:4, 20:5, 22:0, 22:1, 22:4, 22:6, 23:0, 24:0 , 24:1
- Cholesterol synthesis pathway: squalene, lanosterol, desmosterol, lathosterol, cholesterol
- Intestinal absorption markers: phytosterols (campesterol, β-sitosterol, stigmasterol), cholestanol
Matrices: Biofluids (plasma, serum, lipoproteins (VLDL, LDL HDL)), faeces, tissue (liver, adipose tissue, brain, heart, muscle), others (exosomes, mitochondria, cells, bacteria, etc.)
Equipment on the platform
- GC-MS: Trace 1310/ISQ LT (ThermoFisherScientific)
- LC-MS/MS: HPLC (Prominence Shimadzu)/ QTrap 4000 (AB Sciex)
- Pre-analytical automaton LV200 (Biotage)
- Precellys Evolution homogenizer (Ozyme),
- Positive pressure manifold (Waters positive pressure manifold – 96)
- SPD131DDA speed vacuum concentrator with cryogenic trap (Thermo Fisher Scientific).

Current developments
- Sphingosines, sphingosine-1-Phosphate
- Oxysterols
Lipidomics Platform Interaction Radiation
- Education: reference platform for the OMICS course of the Biomics master’s degree from Université Paris Est Créteil
Local networks: UMS RésOmique SU
National networks: MetaboHub, RFMF, Lipidomystes, INSERM Microbiota Consortium
International networks: ELM, MPF, metabomeeting, european regional metabolomics networks
Certificates
Label IBiSA obtenu en 2020 (https://www.ibisa.net/plateformes/icanomics-635.html)
Team
Marie Lhomme (IR, ICAN)
Operational manager
Dr Anatol Kontush (DR, UMR ICAN 1166)
Scientific Director
Maharajah Ponnaiah (IR, ICAN)
data scientist
Sora Lecocq (Tech, ICAN)
Technician
Scientific Committee
Prof. F. Mochel (ICM)
Dr. F. Lamari (APHP)
Dr. G. I. Boneca (Pastor)
Dr F. Fenaille (CEA)
Wilfried Le Goff (UMR ICAN 1166)
Contact
Access conditions
- Download the form below and return to m.lhomme@ihuican.org
- Your project will be submitted to the steering committee
- After validation, estimate drawn up and project initiated